The Optimization of Protease Enzyme Extraction From Streblus Asper (Kesinai)
نویسندگان
چکیده
منابع مشابه
Optimization of the conditions for extraction of serine protease from kesinai plant (Streblus asper) leaves using response surface methodology.
Response surface methodology (RSM) using a central composite design (CCD) was employed to optimize the conditions for extraction of serine protease from kesinai (Streblus asper) leaves. The effect of independent variables, namely temperature (42.5,47.5, X₁), mixing time (2-6 min, X₂), buffer content (0-80 mL, X₃) and buffer pH (4.5-10.5, X₄) on specific activity, storage stability, temperature ...
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A 'Heat treatment aqueous two phase system' was employed for the first time to purify serine protease from kesinai (Streblus asper) leaves. In this study, introduction of heat treatment procedure in serine protease purification was investigated. In addition, the effects of different molecular weights of polyethylene glycol (PEG 4000, 6000 and 8000) at concentrations of 8, 16 and 21% (w/w) as we...
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Aqueous and ethanol extracts of oven and freeze-dried Streblus asper leaves were investigated using DPPH assay. The presence of phenolic compounds and flavonoids in the extracts, which were detected by Folin and colorimetric assays, respectively, may be responsible for the antioxidant activities of S. asper. The different drying treatments resulted in significant differences (p < 0.05) in the a...
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ژورنال
عنوان ژورنال: IOP Conference Series: Materials Science and Engineering
سال: 2020
ISSN: 1757-899X
DOI: 10.1088/1757-899x/932/1/012039